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Ask me anything
Cheesy-GordiaDate: Sunday, 22-May-2011, 12:41 PM | Message # 91
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Fav Genre of music?



I hate this n***a actin like jesus - SOUNDWAVES

You don't are pro - Adam_CZ
 
JohnSmith55Date: Sunday, 22-May-2011, 12:50 PM | Message # 92
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Quote (Hash)
When inserting a vector to a bacteria, many factors come into play for the success of vectorial insertion

well no sh!t sherlock

Quote (Hash)
what technique should we use to guarantee a successful insertion with maximum desired products?

There are no guarantees (100%) for a successful insertion, but out of all the various techniques I would use either plasmid transformation or electroporation to achieve the best results.

Also, you didn't specify whether we are dealing with plant or animal cells so it's still a broad question.

Added (2011-05-22, 1:50 PM)
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Quote (Hash)
PS: I have many more of these kind of questions which are on a more difficult level

I can't wait, just don't throw all your molecular biology test-material at me.

Quote (Cheesy-Gordia)
Fav Genre of music?

That would be Goa trance.

 
ItSoVeR9000Date: Sunday, 22-May-2011, 12:58 PM | Message # 93
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hmm Whats your favorite dish to make?!

 
HashDate: Sunday, 22-May-2011, 1:01 PM | Message # 94
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LOL, how is that too broad? I was talking about bacterial vector (insert in the bacteria) not in a cell.... sad Electroporation does not work on bacterial insertion.... Should I give you another F- for not reading my question? Don't worry, molecular biology will only come toward the end right before Physical Chemistry tongue
 
JohnSmith55Date: Sunday, 22-May-2011, 1:09 PM | Message # 95
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Quote (ItSoVeR9000)
Whats your favorite dish to make?

I'm a total n00b when it comes to cooking.

I can make grits with milk and it's my favorite.
(I'm so gonna get the lulz for this)

Added (2011-05-22, 2:09 PM)
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Should I give you another F- for not reading my question?

Give me an E+ for the effort, my answer came from zero knowledge + 10 minutes.

 
HashDate: Sunday, 22-May-2011, 1:23 PM | Message # 96
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OK, I'll give you a C- for effort. Please re-answer my question.
 
JohnSmith55Date: Sunday, 22-May-2011, 1:51 PM | Message # 97
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Please re-answer my question.

That is a request (phew!) and not a question, so I have the option to deny your request.

I don't feel like re-answering your question at the moment, thanks anyway! biggrin

 
lolbladeDate: Sunday, 22-May-2011, 4:45 PM | Message # 98
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May you plz accept all the requests in this thread that you have said are not questions? biggrin

sig in construction
 
TrixxareforkidsDate: Sunday, 22-May-2011, 5:26 PM | Message # 99
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What are you going to do to celebrate the 100th reply to this thread?

"being admin on a forum is NOT a super power" - lSpiderl

"CHF BECAMED SUCK S" - yan

"CHF has been my childhood. It actually defined lots of my childhood." - RosarioVampireZ
 
JohnSmith55Date: Sunday, 22-May-2011, 10:28 PM | Message # 100
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Quote (lolblade)
May you plz accept all the requests in this thread that you have said are not questions?

Requests are not questions, therefore I cannot accept the heavy burden of answering requests as well.

Quote (Trixxareforkids)
What are you going to do to celebrate the 100th reply to this thread?

sit out on the veranda, grab a beer, invite some friends over, turn up the music, etc...

 
HashDate: Saturday, 28-May-2011, 1:22 PM | Message # 101
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Why are you always trying to confuse me?

Added (2011-05-28, 2:22 PM)
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Also, how do you like it if I slap you silly for giving answers which don't make sense?

 
JohnSmith55Date: Saturday, 28-May-2011, 1:33 PM | Message # 102
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Why are you always trying to confuse me?


You made the false assumption that you're the only one I'm trying to confuse! biggrin

Plus, you asked two questions (which is a terrible mistake) so the 'why' has to wait.

Quote (Hash)
Also, how do you like it if I slap you silly for giving answers which don't make sense?


I like it very much if you slap me since it'll eventually lead to you washing my car...
 
Cheesy-GordiaDate: Saturday, 28-May-2011, 3:23 PM | Message # 103
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How many chicks have you banged?



I hate this n***a actin like jesus - SOUNDWAVES

You don't are pro - Adam_CZ
 
HashDate: Saturday, 28-May-2011, 4:11 PM | Message # 104
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Uhhh NO! I asked you why, not who!
 
JohnSmith55Date: Saturday, 28-May-2011, 10:54 PM | Message # 105
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Quote (Cheesy-Gordia)
How many chicks have you banged?


More than three, less than thirty. wink
 
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